GOPC antibody (C-Term)

Catalog No. ABIN185161

This Goat Polyclonal antibody specifically detects GOPC in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for GOPC antibody (C-Term) (ABIN185161)

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GOPC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-GOPC Antibody

Binding Specificity: C-Term

Purpose: PIST / FIG / GOPC

Sequence: LDDLHTLYHK KSY

Specificity: This antibody is expected to recognize both reported isoforms (NP_065132.1, NP_001017408.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LDDLHTLYHKKSY, from the C Terminus of the protein sequence according to NP_065132.1, NP_001017408.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 55 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 50.5 kDa according to NP_065132.1). Recommended concentration: 0.05-0.2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: This product has been successfully used on HeLa cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GOPC

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Alternative Name: GOPC

Background: PIST, GOPC, CAL, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, fused in glioblastoma, CFTR-associated ligand, Golgi associated PDZ and coiled-coil motif containing protein, golgi associated PDZ and coiled-coil motif containing, CA

Gene ID: 57120

NCBI Accession: NP_065132, NP_001017408

Pathways: Maintenance of Protein Location, Asymmetric Protein Localization