PDLIM4 antibody (C-Term)

Catalog No. ABIN185168

The Goat Polyclonal anti-PDLIM4 antibody has been validated for WB, ELISA and IF. It is suitable to detect PDLIM4 in samples from Human.

Quick Overview for PDLIM4 antibody (C-Term) (ABIN185168)

Target: PDLIM4 (PDZ and LIM Domain 4 (PDLIM4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PDLIM4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-PDLIM4 Antibody

Binding Specificity: C-Term

Purpose: PDLIM4 / RIL

Sequence: DVVAVYPNAK VELV

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DVVAVYPNAKVELV, from the C Terminus of the protein sequence according to NP_003678.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in lysates of cell line A431 (calculated MW of 35.4 kDa according to NP_003678.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PDLIM4

Target: PDLIM4 (PDZ and LIM Domain 4 (PDLIM4))

Alternative Name: PDLIM4

Background: PDLIM4, RIL, LIM domain protein, PDZ and LIM domain 4, enigma homolog

Gene ID: 8572, 30794, 24915

NCBI Accession: NP_003678