VPS29 antibody (C-Term)

Catalog No. ABIN185173

This Goat Polyclonal antibody specifically detects VPS29 in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for VPS29 antibody (C-Term) (ABIN185173)

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS29 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-VPS29 Antibody

Binding Specificity: C-Term

Purpose: VPS29 (C Terminus)

Sequence: DDVKVERIEY KKP

Specificity: This antibody is expected to recognise both human isoforms (represented by NP_057310.1, NP_476528.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DDVKVERIEYKKP, from the C Terminus of the protein sequence according to NP_057310.1, NP_476528.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 20+21 kDa bands observed in Human Spleen lysates (calculated MW of 20.5 kDa according to NP_057310.1 and 20.9 kDa according to NP_476528.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubated for 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VPS29

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Alternative Name: VPS29

Background: VPS29, DC7, DC15, PEP11, FLJ20492, DKFZp564F0223, vacuolar protein sorting 29 (yeast), x 007 protein, retromer protein, vacuolar sorting protein VPS29/PEP11, vacuolar protein sorting 29 (yeast homolog), vacuolar protein sorting 29 homolog (S. cerevisiae),

Gene ID: 51699, 56433

NCBI Accession: NP_057310, NP_476528