Transgelin antibody (C-Term)

Catalog No. ABIN185188

This Goat Polyclonal antibody specifically detects Transgelin in WB, ELISA and IHC. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for Transgelin antibody (C-Term) (ABIN185188)

Target: Transgelin (TAGLN)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Transgelin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Transgelin Antibody

Binding Specificity: C-Term

Purpose: SM22 Alpha / Transgelin

Sequence: MTGYGRPRQI IS

Specificity: NP_003177.2 and NP_001001522.1 represent identical protein. The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earli

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-MTGYGRPRQIIS

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 23-24 kDa band observed in Human Testis lysates (calculated MW of 22.6 kDa according to NP_003177.2 and NP_001001522.1). Preliminary testing also showed a band at approx 23 kDa in lysates of cell line HeLa and approx 25 kDa in Human Brea

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Transgelin antibody (ABIN185188)

Donati, Marseglia, Magi, Serratì, Cencetti, Bernacchioni, Nannetti, Benelli, Brunelli, Torricelli, Cossu, Bruni: "Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6." in: PLoS ONE, Vol. 6, Issue 5, pp. e20389, (2011) (PubMed).

Cencetti, Bernacchioni, Nincheri, Donati, Bruni: "Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis." in: Molecular biology of the cell, Vol. 21, Issue 6, pp. 1111-24, (2010) (PubMed).

Nincheri, Luciani, Squecco, Donati, Bernacchioni, Borgognoni, Luciani, Benvenuti, Francini, Bruni: "Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 10, pp. 1741-54, (2009) (PubMed).

Donoghue, Doran, Wynne, Pedersen, Dunn, Ohlendieck: "Proteomic profiling of chronic low-frequency stimulated fast muscle." in: Proteomics, Vol. 7, Issue 18, pp. 3417-30, (2007) (PubMed).

Target Details for Transgelin

Target: Transgelin (TAGLN)

Alternative Name: TAGLN

Background: TAGLN, SM22, SMCC, WS3-10, transgelin, SM22-alpha, DKFZp686P11128, TAGLN1, DKFZp686B01212, transgelin variant 2

Gene ID: 6876, 21345, 25123

NCBI Accession: NP_003177