ACTR1A antibody (C-Term)

Catalog No. ABIN185219

The Goat Polyclonal anti-ACTR1A antibody has been validated for WB, ELISA and FACS. It is suitable to detect ACTR1A in samples from Human, Mouse, Rat and Dog.

Quick Overview for ACTR1A antibody (C-Term) (ABIN185219)

Target: ACTR1A (Alpha Centractin (ACTR1A))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ACTR1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ACTR1A Antibody

Binding Specificity: C-Term

Purpose: ARP1 homolog A

Sequence: YEEDGARSIH RKT

Specificity: This epitope was selected to minimise the chance of cross-reactivity with ACTR1B (YEEDGSRAIHRKT).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YEEDGARSIHRKT, from the C Terminus of the protein sequence according to NP_005727.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 42-45 kDa band observed in lysates of cell lines A431 HEK293, HeLa, MCF7, NIH3T3, KNRK and MDCK, and in preliminary testing of Human and Mouse Adrenal gland and Mouse Stomach lysate (calculated MW of 42.6 kDa according to Human NP_005

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: A customer reported cytoplasmic staining on A431 cells at a primary ab concentration of 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACTR1A

Target: ACTR1A (Alpha Centractin (ACTR1A))

Alternative Name: ACTR1A

Background: ACTR1A, ARP1, ARP1 actin-related protein 1 homolog A, centractin alpha (yeast), actin-RPV, centractin alpha, ARP1, yeast homolog A, centrosome-associated actin homolog, ARP1 (actin-related protein 1, yeast) homolog A (centractin alpha), CTRN1, FLJ52695, F

Gene ID: 10121, 54130

NCBI Accession: NP_005727

Pathways: M Phase