FTCD antibody (Internal Region)

Catalog No. ABIN185268

This anti-FTCD antibody is a Goat Polyclonal antibody detecting FTCD in WB, ELISA, IF and FACS. Suitable for Human, Mouse and Pig.

Quick Overview for FTCD antibody (Internal Region) (ABIN185268)

Target: FTCD (Formiminotransferase Cyclodeaminase (FTCD))

Reactivity: Human, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FTCD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-FTCD Antibody

Binding Specificity: Internal Region

Purpose: 58KGolgi protein(Internal)/FTCD

Sequence: CLREQGRGKD QPGRL

Specificity: Variants (NP_006648.1, NP_996848.1) encode the same protein.

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CLREQGRGKDQPGRL, from the internal region of the protein sequence according to NP_006648.1, NP_996848.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human and Mouse Liver lysates and approx. 55-60 kDa in Pig Liver lysates, and also in preliminary testing of Rat Liver and Human Kidney lysates (calculated MW of 58.9 kDa according to Human NP_006648.1, Mouse NP_

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membranes of HeLa and in the membranes of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FTCD

Target: FTCD (Formiminotransferase Cyclodeaminase (FTCD))

Alternative Name: FTCD

Background: FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase, LC1 autoantigen

Gene ID: 10841

NCBI Accession: NP_006648, NP_996848, NP_001307341