PTP4A1 antibody (C-Term)

Catalog No. ABIN185274

Cited in 2+ publications. The Goat Polyclonal anti-PTP4A1 antibody (ABIN185274) specifically detects PTP4A1 in WB, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for PTP4A1 antibody (C-Term) (ABIN185274)

Target: PTP4A1 (Protein Tyrosine Phosphatase Type IVA, Member 1 (PTP4A1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PTP4A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-PTP4A1 Antibody

Binding Specificity: C-Term

Purpose: PRL1 / PTP4A1

Sequence: CFKDSNGHRN N

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CFKDSNGHRNN, from the C Terminus of the protein sequence according to NP_003454.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: This antibody has been successfully used in Western Blot as descibed in the following papers, PMID: 18997816 and PMID: 36793871.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in HeLa and NIH3T3 cells. Recommended concentration: 10μg/ml.

Additional validation: This antibody has been successfully used in the following paper: Achiwa H e

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PTP4A1 antibody (ABIN185274)

Min, Kim, Heo, Kim, Kim, Kim, Han, Kim, Yoo: "New p53 target, phosphatase of regenerating liver 1 (PRL-1) downregulates p53." in: Oncogene, Vol. 28, Issue 4, pp. 545-54, (2009) (PubMed).

Achiwa, Lazo: "PRL-1 tyrosine phosphatase regulates c-Src levels, adherence, and invasion in human lung cancer cells." in: Cancer research, Vol. 67, Issue 2, pp. 643-50, (2007) (PubMed).

Target Details for PTP4A1

Target: PTP4A1 (Protein Tyrosine Phosphatase Type IVA, Member 1 (PTP4A1))

Alternative Name: PTP4A1

Background: PTP4A1, HH72, PRL1, PRL-1, PTPCAAX1, PTP(CAAX1), protein tyrosine phosphatase type IVA, member 1, Protein tyrosine phosphatase IVA1, RP5-1148A21.2, DKFZp779M0721, protein tyrosine phosphatase type IVA protein 1

Gene ID: 7803

NCBI Accession: NP_003454