DKK1 antibody (C-Term)

Catalog No. ABIN185275

This Goat Polyclonal antibody specifically detects DKK1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for DKK1 antibody (C-Term) (ABIN185275)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DKK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-DKK1 Antibody

Binding Specificity: C-Term

Purpose: DKK1

Sequence: CDHHQASNSS RLHT

Cross-Reactivity: Cow, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CDHHQASNSSRLHT, from the C Terminus of the protein sequence according to NP_036374.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 10 μg/mL.

Western Blot: Approx 38 kDa band observed in Human Bone Marrow and Spleen lysates (calculated MW of 28.7 kDa according to NP_036374.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fedi et al, J Bio

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1

Background: DKK1, SK, DKK-1, dickkopf homolog 1 (Xenopus laevis), dickkopf-1 like, dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1, dickkopf related protein-1

Gene ID: 22943

NCBI Accession: NP_036374

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation