VAV3 antibody (Internal Region)

Catalog No. ABIN185278

This Goat Polyclonal antibody specifically detects VAV3 in ELISA, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for VAV3 antibody (Internal Region) (ABIN185278)

Target: VAV3 (Vav 3 Oncogene (VAV3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VAV3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-VAV3 Antibody

Binding Specificity: Internal Region

Purpose: VAV3

Sequence: C-SGEQGTLKLP EK

Specificity: This antibody is expected to recognize both reported isoforms (NP_006104.4, NP_001073343.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CSGEQGTLKLPEK, from the internal region of the protein sequence according to NP_006104.4, NP_001073343.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 7.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VAV3

Target: VAV3 (Vav 3 Oncogene (VAV3))

Alternative Name: VAV3

Background: VAV3, vav 3 oncogene, vav 3 guanine nucleotide exchange factor, FLJ40431

Gene ID: 10451

NCBI Accession: NP_006104, NP_001073343

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway