TXNDC5 antibody (C-Term)

Catalog No. ABIN185279

The Goat Polyclonal anti-TXNDC5 antibody has been validated for WB, ELISA, IHC, IF and FACS. It is suitable to detect TXNDC5 in samples from Human.

Quick Overview for TXNDC5 antibody (C-Term) (ABIN185279)

Target: TXNDC5 (Thioredoxin Domain Containing 5 (Endoplasmic Reticulum) (TXNDC5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TXNDC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-TXNDC5 Antibody

Binding Specificity: C-Term

Purpose: EndoPDI / TXNDC5

Sequence: SLHRFVLSQA KDEL

Specificity: This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437.2, NP_001139021.1.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SLHRFVLSQAKDEL, from the C Terminus of the protein sequence according to NP_110437.2, NP_001139021.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in lysates of cell lines HEK293, A549, HeLa and HepG2 (calculated MW of 47.6 kDa according to NP_110437.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-0.1 μg/mL. Pr

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended con

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TXNDC5

Target: TXNDC5 (Thioredoxin Domain Containing 5 (Endoplasmic Reticulum) (TXNDC5))

Alternative Name: TXNDC5

Background: TXNDC5, EndoPDI, thioredoxin domain containing 5, ERP46, UNQ364, MGC3178, FLJ21353, FLJ90810, thioredoxin related protein, endothelial protein disulphide, thioredoxin domain containing 5 (endoplasmic reticulum) isomerase, Hcc-2, PDIA15, endothelial protei

Gene ID: 81567

NCBI Accession: NP_110437, NP_001139021

Pathways: Cell RedoxHomeostasis