SCARF1 antibody (AA 20-31)

Catalog No. ABIN185285

The Goat Polyclonal anti-SCARF1 antibody has been validated for WB and ELISA. It is suitable to detect SCARF1 in samples from Human.

Quick Overview for SCARF1 antibody (AA 20-31) (ABIN185285)

Target: SCARF1 (Acetyl LDL Receptor (SCARF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SCARF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SCARF1 Antibody

Binding Specificity: AA 20-31

Purpose: SCARF1 / SREC

Sequence: SELDPKGQHV C

Specificity: This antibody is expected to recognise all three reported isoforms (NP_003684.2, NP_663325.1, NP_663327.2). Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 / SREC1 protein.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SELDPKGQHVC, from the internal region of the protein sequence according to NP_003684.2, NP_663325.1, NP_663327.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 85-90 kDa band observed in Human Heart lysates (calculated MW of 87.4 kDa according to NP_003684.2). Recommended concentration: 0.3-1.0 μg/mL. Primary incubation was 1 hour. An additional faint band of 28 kDa was consistently observed,

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SCARF1

Target: SCARF1 (Acetyl LDL Receptor (SCARF1))

Alternative Name: SCARF1

Background: SCARF1, SREC, KIAA0149, MGC47738, scavenger receptor class F, member 1, acetyl LDL receptor, scavenger receptor expressed by endothelial cells

Gene ID: 8578

NCBI Accession: NP_003684, NP_663325, NP_663327