GSTM1 antibody (C-Term)

Catalog No. ABIN185334

This anti-GSTM1 antibody is a Goat Polyclonal antibody detecting GSTM1 in WB and ELISA. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for GSTM1 antibody (C-Term) (ABIN185334)

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GSTM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GSTM1 Antibody

Binding Specificity: C-Term

Purpose: GSTM1 / GSTM2

Sequence: CVFSKMAVWG NK

Specificity: This antibody is expected to recognise both reported variants of human GSTM1 protein (NP_000552.2, NP_666533.1) and wil also recognise human GSTM2 protein (NP_000839.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CVFSKMAVWGNK, from the C Terminus of the protein sequence according to NP_000552.2, NP_666533.1, NP_000839.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26 kDa band observed in Human Liver and Human Lung lysates (calculated MW of 25.7 kDa according to NP_000552.2, and NP_000839.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-GSTM1 antibody (ABIN185334)

Endo, Uno, Seki, Ariga, Kusumi, Mitsumata, Yamada, Makishima: "Inhibition of aryl hydrocarbon receptor transactivation and DNA adduct formation by CYP1 isoform-selective metabolic deactivation of benzo[a]pyrene." in: Toxicology and applied pharmacology, Vol. 230, Issue 2, pp. 135-43, (2008) (PubMed).

Target Details for GSTM1

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Alternative Name: GSTM1 / GSTM2

Background: GSTM1, GSTM2, MU, H-B, GST1, GTH4, GTM1, MU-1, GSTM1-1, MGC26563, GSTM1a-1a, GSTM1b-1b, glutathione S-transferase M1, HB subunit 4, GST class-mu 1, glutathione S-aryltransferase, glutathione S-alkyltransferase, glutathione S-transferase, Mu-1, glutathione

Gene ID: 2944, 2946

NCBI Accession: NP_000552, NP_666533, NP_000839

Pathways: Negative Regulation of Transporter Activity