Cytochrome B Reductase 1 antibody (C-Term)

Catalog No. ABIN185343

This anti-Cytochrome B Reductase 1 antibody is a Goat Polyclonal antibody detecting Cytochrome B Reductase 1 in ELISA, WB and IHC. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for Cytochrome B Reductase 1 antibody (C-Term) (ABIN185343)

Target: Cytochrome B Reductase 1 (CYBRD1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Cytochrome B Reductase 1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Cytochrome B Reductase 1 Antibody

Binding Specificity: C-Term

Purpose: Cytochrome b reductase 1

Sequence: CRNLALDEAG QRSTM

Specificity: This antibody is expected to recognise isoform 1 (NP_079119.3) only.

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CRNLALDEAGQRSTM, from the C Terminus of the protein sequence according to NP_079119.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Small Intestine. Rec

Western Blot: Preliminary experiments gave bands at approx 35 kDa and a 26+28 kDa doublet in human colon lysate after 0.1 μg/mL antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfull

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Cytochrome B Reductase 1 antibody (ABIN185343)

Dostalikova-Cimburova, Balusikova, Kratka, Chmelikova, Hejda, Hnanicek, Neubauerova, Vranova, Kovar, Horak: "Role of duodenal iron transporters and hepcidin in patients with alcoholic liver disease." in: Journal of cellular and molecular medicine, Vol. 18, Issue 9, pp. 1840-50, (2014) (PubMed).

Dostalikova-Cimburova, Kratka, Balusikova, Chmelikova, Hejda, Hnanicek, Neubauerova, Vranova, Kovar, Horak: "Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency." in: Journal of cellular and molecular medicine, Vol. 16, Issue 8, pp. 1816-26, (2012) (PubMed).

Balusikova, Neubauerova, Dostalikova-Cimburova, Horak, Kovar: "Differing expression of genes involved in non-transferrin iron transport across plasma membrane in various cell types under iron deficiency and excess." in: Molecular and cellular biochemistry, Vol. 321, Issue 1-2, pp. 123-33, (2009) (PubMed).

Target Details for Cytochrome B Reductase 1

Target: Cytochrome B Reductase 1 (CYBRD1)

Alternative Name: CYBRD1

Background: CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b, FRRS3, ferric-chelate reductase 3

Gene ID: 79901

NCBI Accession: NP_079119

Pathways: Transition Metal Ion Homeostasis