SRD5A2 antibody (N-Term)

Catalog No. ABIN185347

This Goat Polyclonal antibody specifically detects SRD5A2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for SRD5A2 antibody (N-Term) (ABIN185347)

Target: SRD5A2 (Steroid-5-alpha-Reductase, alpha Polypeptide 2 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 2) (SRD5A2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SRD5A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SRD5A2 Antibody

Binding Specificity: N-Term

Purpose: SRD5A2 / 5-alpha reductase 2

Sequence: QVQCQQSPVL AG

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QVQCQQSPVLAG, from the N Terminus of the protein sequence according to NP_000339.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26 kDa band observed in Human Prostate and Breast lysates (calculated MW of 28.4 kDa according to NP_000339.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SRD5A2

Target: SRD5A2 (Steroid-5-alpha-Reductase, alpha Polypeptide 2 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 2) (SRD5A2))

Alternative Name: SRD5A2

Background: SRD5A2, steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2), steroid 5-alpha-reductase 2, MGC138457, 3-oxo-5 alpha-steroid 4-dehydrogenase 2

Gene ID: 6716

NCBI Accession: NP_000339

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis