DYX1C1 antibody (C-Term)

Catalog No. ABIN185356

This Goat Polyclonal antibody specifically detects DYX1C1 in WB, ELISA and IHC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for DYX1C1 antibody (C-Term) (ABIN185356)

Target: DYX1C1 (Dyslexia Susceptibility 1 Candidate 1 (DYX1C1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DYX1C1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-DYX1C1 Antibody

Binding Specificity: C-Term

Purpose: DYX1C1 (Isoform a)

Sequence: KIRNVIQGTE LKS

Specificity: This antibody is expected to recognise only one of the three reported isoforms (NP_570722.2, isoform a).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KIRNVIQGTELKS, from the C Terminus of the protein sequence according to NP_570722.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in some of the neuronal cells. Recommended concentration, 5-10 μg/mL.

Western Blot: Lysates of COS1 transfected with full length recombinant Human DYX1C1 gave bands at approx 48 kDa after 0.1 μg/mL antibody staining. In addition, a minor band of 24 kDa is detected consistent with observations with N-terminal specific antibod

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-DYX1C1 antibody (ABIN185356)

Wang, Paramasivam, Thomas, Bai, Kaminen-Ahola, Kere, Voskuil, Rosen, Galaburda, Loturco: "DYX1C1 functions in neuronal migration in developing neocortex." in: Neuroscience, Vol. 143, Issue 2, pp. 515-22, (2006) (PubMed).

Target Details for DYX1C1

Target: DYX1C1 (Dyslexia Susceptibility 1 Candidate 1 (DYX1C1))

Alternative Name: DYX1C1

Background: DYX1C1, DYX1, EKN1, DYXC1, FLJ37882, dyslexia susceptibility 1 candidate 1, MGC70618, RD

Gene ID: 161582, 67685

NCBI Accession: NP_570722

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling