Asporin antibody (Internal Region)

Catalog No. ABIN185364

This anti-Asporin antibody is a Goat Polyclonal antibody detecting Asporin in WB, ELISA and IF. Suitable for Human, Mouse and Rat.

Quick Overview for Asporin antibody (Internal Region) (ABIN185364)

Target: Asporin (ASPN)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Asporin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-Asporin Antibody

Binding Specificity: Internal Region

Purpose: Asporin / ASPN

Sequence: IHENKVKKIQ KDT

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-IHENKVKKIQKDT, from the internal region of the protein sequence according to NP_060150.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Human Tonsil and Uterus and in Mouse and Rat Skeletal Muscle lysates (calculated MW of 43.3 kDa according to Human NP_060150.4, and 42.6 kDa acciording to Mouse NP_001165952.1 and Rat NP_001014030.1). Recommende

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Asporin

Target: Asporin (ASPN)

Alternative Name: ASPN

Background: ASPN, PLAP1, SLRR1C, FLJ20129, asporin (LRR class 1), small leucine-rich protein 1C, periodontal ligament associated protein 1, asporin, OS3, asporin proteoglycan

Gene ID: 54829

NCBI Accession: NP_060150