RBP1 antibody (C-Term)

Catalog No. ABIN185365

This Goat Polyclonal antibody specifically detects RBP1 in WB, IF, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for RBP1 antibody (C-Term) (ABIN185365)

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-RBP1 Antibody

Binding Specificity: C-Term

Purpose: RBP1

Sequence: VEGVVCKQVF KKVQ

Cross-Reactivity: Cow, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence VEGVVCKQVFKKVQ, from the C Terminus of the protein sequence according to NP_002890.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 16 kDa band observed in NIH3T3 and approx 17 kDa in U251 cell lysates. This molecular weight is routinely observed by other sources. (calculated MW of 22.3 kDa according to Human NP_002890.2 and 15.8 kDa according to Mouse NP_035384.1).

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RBP1

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Alternative Name: RBP1

Background: RBP1, CRBP, RBPC, CRBP1, CRABP-I, retinol binding protein 1, cellular, retinol-binding protein 1, cellular, CRBPI

Gene ID: 5947

NCBI Accession: NP_002890