Retinoic Acid Receptor alpha antibody (C-Term)

Catalog No. ABIN185366

The Goat Polyclonal anti-Retinoic Acid Receptor alpha antibody has been validated for WB and ELISA. It is suitable to detect Retinoic Acid Receptor alpha in samples from Human.

Quick Overview for Retinoic Acid Receptor alpha antibody (C-Term) (ABIN185366)

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Retinoic Acid Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Retinoic Acid Receptor alpha Antibody

Binding Specificity: C-Term

Purpose: RARA

Sequence: SPSLSPSSHR SSPATQSP

Specificity: This antibody is expected to recognize all three reported isoforms (NP_000955.1, NP_001019980.1, NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. Reported variants NP_0

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SPSLSPSSHRSSPATQSP, from the C Terminus of the protein sequence according to NP_000955.1, NP_001138773.1, NP_001019980.1, NP_001138774.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Breast and Breast cancer lysates (calculated MW of 50.8 kDa according to NP_000955.1and NP_001019980.1). Recommended concentration: 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Retinoic Acid Receptor alpha

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Alternative Name: RARA

Background: RARA, RAR, NR1B1, retinoic acid receptor, alpha, Retinoic acid receptor, alpha polypeptide, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR short form

Gene ID: 5914

NCBI Accession: NP_000955, NP_001138773, NP_001019980, NP_001138774

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, S100 Proteins