PUM2 antibody (Internal Region)

Catalog No. ABIN185369

The Goat Polyclonal anti-PUM2 antibody has been validated for WB, ELISA and IHC. It is suitable to detect PUM2 in samples from Human.

Quick Overview for PUM2 antibody (Internal Region) (ABIN185369)

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PUM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PUM2 Antibody

Binding Specificity: Internal Region

Purpose: PUM2

Sequence: DQNRDLKQGD DDDSK

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DQNRDLKQGDDDDSK, from the internal region of the protein sequence according to NP_056132.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 10 μg/mL.

Western Blot: In transfected HEK293 transiently expressing full-length Human PUM2 (myc and DYKDDDDK tagged), a band of approx. 140 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag a

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PUM2

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Alternative Name: PUM2

Background: PUM2, PUMH2, PUML2, KIAA0235, pumilio homolog 2 (Drosophila), pumilio (Drosphila) homolog 2, FLJ36528, MGC138251, MGC138253, OTTHUMP00000200539, pumilio homolog 2

Gene ID: 23369

NCBI Accession: NP_056132

Pathways: Ribonucleoprotein Complex Subunit Organization