POMC antibody (C-Term)

Catalog No. ABIN185387

The Goat Polyclonal anti-POMC antibody has been validated for ELISA, IHC and FACS. It is suitable to detect POMC in samples from Human.

Quick Overview for POMC antibody (C-Term) (ABIN185387)

Target: POMC (Proopiomelanocortin (POMC))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This POMC antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-POMC Antibody

Binding Specificity: C-Term

Purpose: Proopiomelanocortin / POMC

Sequence: NAIIKNAYKK GE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NAIIKNAYKKGE, from the C Terminus of the protein sequence according to NP_000930.1, NP_001030333.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Anterior Pituitary Gland shows vesilulate staining in glandular cells. Recommended concentration: 2-4 μg/mL.

Western Blot: Preliminary testing showed a band at approx 30 kDa in A431 cell lysate after 1 μg/mL antibody staining (calculated MW of 29.4 kDa according to NP_000930.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for POMC

Target: POMC (Proopiomelanocortin (POMC))

Alternative Name: POMC

Background: POMC, proopiomelanocortin, Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating, hormone/ beta-endorphin, LPH, NPP, OTTHUMP00000119991, OTTHUMP00000200964, adrenocortic

Gene ID: 5443

NCBI Accession: NP_000930, NP_001030333

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour