DDC antibody (C-Term)

Catalog No. ABIN185393

The Goat Polyclonal anti-DDC antibody has been validated for WB and ELISA. It is suitable to detect DDC in samples from Human.

Quick Overview for DDC antibody (C-Term) (ABIN185393)

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DDC antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DDC Antibody

Binding Specificity: C-Term

Purpose: DOPA decarboxylase

Sequence: WEHIKELAAD VL

Specificity: This antibody is expected to recognise isoforms 1, 2, 3, 4 and 5 (NP_000781.1, NP_001229815.1, NP_001229816.1, NP_001229817.1, NP_001229818.1 respectively). Reported variants represent identical protein (NP_000781.1, NP_001076440.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-WEHIKELAADVL, from the C Terminus of the protein sequence according to NP_000781.1, NP_001229815.1, NP_001229816.1, NP_001229817.1, NP_001229818.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Human Kidney lysates and 48 kDa on Human Brain lysates (calculated MW of 53.9 kDa according to NP_000781.1). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DDC

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Alternative Name: DDC

Background: DDC, dopa decarboxylase (aromatic L-amino acid decarboxylase) , HGNC:2719, AADC, aromatic L-amino acid decarboxylase

Gene ID: 1644

NCBI Accession: NP_000781, NP_001229815, NP_001229816, NP_001229817, NP_001229818

Pathways: Dopaminergic Neurogenesis