RNLS antibody (Internal Region)

Catalog No. ABIN185396

The Goat Polyclonal anti-RNLS antibody has been validated for WB and ELISA. It is suitable to detect RNLS in samples from Human, Mouse and Rat.

Quick Overview for RNLS antibody (Internal Region) (ABIN185396)

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This RNLS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-RNLS Antibody

Binding Specificity: Internal Region

Purpose: Renalase (aa 224 to 233)

Sequence: VSIDNKKRNI

Specificity: This antibody is expected to recognise both reported isoforms (NP_001026879.2 and NP_060833.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-VSIDNKKRNI, from the internal region of the protein sequence according to NP_001026879.2, NP_060833.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38+35 kDa bands observed in Human Heart lysates, 37 kDa band observed in Mouse Heart lysates and approx 35 kDa in Rat Heart lysates (calculated MW of 37.8 and 34.9 according to Human NP_001026879.2 and NP_060833.1 resp, of 33.3 kDa accor

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RNLS

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Alternative Name: RNLS

Background: RNLS, renalase, C10orf59, chromosome 10 open reading frame 59 , HGNC:25641, FLJ11218 , hypothetical protein LOC55328, renalase, FAD-dependent amine oxidase, RENALASE

Gene ID: 55328

NCBI Accession: NP_001026879, NP_060833