Lipocalin 2 antibody (Internal Region)

Catalog No. ABIN185405

The Goat Polyclonal anti-Lipocalin 2 antibody has been validated for WB, ELISA and IHC. It is suitable to detect Lipocalin 2 in samples from Human.

Quick Overview for Lipocalin 2 antibody (Internal Region) (ABIN185405)

Target: Lipocalin 2 (LCN2)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Lipocalin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Lipocalin 2 Antibody

Binding Specificity: Internal Region

Purpose: Lipocalin 2 / NGAL

Sequence: AILREDKDPQ KMY

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-AILREDKDPQKMY, from the internal region of the protein sequence according to NP_005555.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells. Recommended concentration: 2-3 μg/mL.

Western Blot: Approx 22 kDa band observed in Human Kidney lysates (calculated MW of 22.6 kDa according to NP_005555.2). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Lipocalin 2

Target: Lipocalin 2 (LCN2)

Alternative Name: LCN2

Background: LCN2, NGAL, lipocalin 2 (oncogene 24p3), HGNC:6526, uterocalin, oncogenic lipocalin 24p3, neutrophil gelatinase-associated lipocalin, lipocalin 2, MSFI, OTTHUMP00000022240, migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin

Gene ID: 3934

NCBI Accession: NP_005555

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis