GSTP1 antibody (Internal Region)

Catalog No. ABIN185418

This anti-GSTP1 antibody is a Goat Polyclonal antibody detecting GSTP1 in WB, IHC, ELISA, IF and FACS. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for GSTP1 antibody (Internal Region) (ABIN185418)

Target: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GSTP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GSTP1 Antibody

Binding Specificity: Internal Region

Purpose: GST3 / GSTP1

Sequence: LADQGQSWKE EV

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LADQGQSWKEEV, from the internal region of the protein sequence according to NP_000843.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 25 kDa band observed in Human Kidney, Lung and Thymus lysates, lysates of cell lines HeLa, HEK293, Jurkat, CaCo-2, K562, NIH3T3, KNRK and MDCK, and, in nuclear lysates of cell lines HeLa, HEK293 and Jurkat (calculated MW of 23.4 kDa ac

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GSTP1

Target: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Alternative Name: GSTP1

Background: GSTP1, GST3, glutathione S-transferase pi , HGNC:4638, DFN7, FAEES3, PI, deafness, X-linked 7, fatty acid ethyl ester synthase III, glutathione transferase, glutathione S-transferase pi 1

Gene ID: 2950

NCBI Accession: NP_000843

Pathways: Cellular Response to Molecule of Bacterial Origin