GSTP1 antibody (Internal Region)

Catalog No. ABIN185418

The Goat Polyclonal anti-GSTP1 antibody (ABIN185418) specifically detects GSTP1 in WB, IHC, ELISA, IF and FACS. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for GSTP1 antibody (Internal Region) (ABIN185418)

Target: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GSTP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GSTP1 Antibody

Binding Specificity: Internal Region

Purpose: GST3 / GSTP1

Sequence: LADQGQSWKE EV

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LADQGQSWKEEV, from the internal region of the protein sequence according to NP_000843.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 25 kDa band observed in Human Kidney, Lung and Thymus lysates, lysates of cell lines HeLa, HEK293, Jurkat, CaCo-2, K562, NIH3T3, KNRK and MDCK, and, in nuclear lysates of cell lines HeLa, HEK293 and Jurkat (calculated MW of 23.4 kDa ac

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GSTP1

Target: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Alternative Name: GSTP1

Background: GSTP1, GST3, glutathione S-transferase pi , HGNC:4638, DFN7, FAEES3, PI, deafness, X-linked 7, fatty acid ethyl ester synthase III, glutathione transferase, glutathione S-transferase pi 1

Gene ID: 2950

NCBI Accession: NP_000843

Pathways: Cellular Response to Molecule of Bacterial Origin