Caveolin-1 antibody (Internal Region)

Catalog No. ABIN185421

This anti-Caveolin-1 antibody is a Goat Polyclonal antibody detecting Caveolin-1 in WB, IHC, ELISA, IF and FACS. Suitable for Human.

Quick Overview for Caveolin-1 antibody (Internal Region) (ABIN185421)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Caveolin-1 Antibody

Binding Specificity: Internal Region

Purpose: Caveolin 1

Sequence: DELSEKQVYD AH

Specificity: This antibody is expected to recognize both reported isoforms (NP_001744.2, NP_001166366.1). Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DELSEKQVYDAH, from the internal region of the protein sequence according to NP_001744.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 22 kDa band observed in lysates of cell lines A431 and A549 and in Human Heart lysates (calculated MW of 20.5 kDa according to NP_001744.2). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at room temperature. Pre

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of A431 cells and in the cytoplasm and Golgi of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analy

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: CAV1

Background: CAV1, caveolin 1, caveolae protein, 22 kDa , HGNC:1527, CAV, MSTP085, VIP21, caveolae protein, 22-kD, caveolin 1, caveolin 1 caveolae protein, 22kD, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32, BSCL3, CGL3, MSTP08

Gene ID: 857, 12389, 25404

NCBI Accession: NP_001744, NP_001166366

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals