SH2D1A antibody (Internal Region)

Catalog No. ABIN185426

The Goat Polyclonal anti-SH2D1A antibody has been validated for WB, ELISA and IP. It is suitable to detect SH2D1A in samples from Human.

Quick Overview for SH2D1A antibody (Internal Region) (ABIN185426)

Target: SH2D1A (SH2 Domain Containing 1A (SH2D1A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SH2D1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-SH2D1A Antibody

Binding Specificity: Internal Region

Purpose: SH2D1A / SAP

Sequence: HKRYFRKIKN

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HKRYFRKIKN, from the internal region of the protein sequence according to NP_002342.1, NP_001108409.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 16 kDa band observed in Human Thymus lysates (calculated MW of 14.2x kDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 16 kDa is observed. This band is not observed in the non-transfect

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SH2D1A

Target: SH2D1A (SH2 Domain Containing 1A (SH2D1A))

Alternative Name: SH2D1A

Background: SH2D1A, SAP, SH2 domain protein 1A, Duncan's disease (lymphoproliferative syndrome), HGNC:10820, DSHP, EBVS, IMD5, LYP, MTCP1, XLP, XLPD , SH2 domain protein 1A, SLAM (signaling lymphocyte activation molecule) associated protein, RP5-1052M9.3, FLJ18687,

Gene ID: 4068

NCBI Accession: NP_002342, NP_001108409

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process