SP1 antibody (Internal Region)

Catalog No. ABIN185433

This anti-SP1 antibody is a Goat Polyclonal antibody detecting SP1 in WB and ELISA. Suitable for Human.

Quick Overview for SP1 antibody (Internal Region) (ABIN185433)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SP1 Antibody

Binding Specificity: Internal Region

Purpose: SP1

Sequence: CSRIESPNEN SNNSQ

Specificity: This antibody is expected to recognise both reported isoforms (NP_612482.2, NP_003100.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CSRIESPNENSNNSQ, from the internal region of the protein sequence according to NP_612482.2, NP_003100.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in lysates of cell lines HeLa (calculated MW of 80.7 kDa according to NP_612482.2). Recommended concentration: 0.3-1 μg/mL. Primary incubated for 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: SP1

Background: SP1, Sp1 transcription factor, HGNC:11205, specificity protein 1

Gene ID: 6667

NCBI Accession: NP_612482, NP_003100

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction