RAE1 antibody (C-Term)

Catalog No. ABIN185434

This Goat Polyclonal antibody specifically detects RAE1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for RAE1 antibody (C-Term) (ABIN185434)

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RAE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-RAE1 Antibody

Binding Specificity: C-Term

Purpose: RAE1

Sequence: NAAEELKPRN KK

Specificity: NP_003601and NP_001015885 represent identical proteins.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NAAEELKPRNKK, from the C Terminus of the protein sequence according to NP_003601.1, NP_001015885.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain Cortex shows staining of neuronal cell bodies. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Heart. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates cell lines HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41.0 kDa according to NP_003601.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RAE1

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Alternative Name: RAE1

Background: RAE1, RAE1 RNA export 1 homolog (S. pombe) , HGNC:9828, FLJ30608, MIG14, MRNP41, Mnrp41, dJ481F12.3, dJ800J21.1, RAE1 (RNA export 1, S.pombe) homolog, homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer), mRNA export protein, mRNA-

Gene ID: 8480

NCBI Accession: NP_003601, NP_001015885

Pathways: SARS-CoV-2 Protein Interactome