DAZL antibody (Internal Region)

Catalog No. ABIN185441

The Goat Polyclonal anti-DAZL antibody has been validated for WB and ELISA. It is suitable to detect DAZL in samples from Rat.

Quick Overview for DAZL antibody (Internal Region) (ABIN185441)

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This DAZL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DAZL Antibody

Binding Specificity: Internal Region

Purpose: DAZL

Sequence: GNGPQKKSVD R

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GNGPQKKSVDR, from the internal region of the protein sequence according to NP_001342.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in Rat Testis lysates (calculated MW of 31.4 kDa according to NP_001102884.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-1 μg/mL. Primary incubation was 1 hour. Pre

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DAZL

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Alternative Name: DAZL

Background: DAZL, deleted in azoospermia-like , HGNC:2685, DAZH, DAZL1, DAZLA, MGC26406, SPGYLA, deleted in azoospermia-like autosomal, germline specific RNA binding protein, spermatogenesis gene on the Y-like autosomal

Gene ID: 1618

NCBI Accession: NP_001342