Ghrelin antibody (C-Term)

Catalog No. ABIN185450

The Goat Polyclonal anti-Ghrelin antibody has been validated for ELISA, IHC and ICC. It is suitable to detect Ghrelin in samples from Human and Rat. There is 1 publication available.

Quick Overview for Ghrelin antibody (C-Term) (ABIN185450)

Target: Ghrelin (GHRL)

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Ghrelin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-Ghrelin Antibody

Binding Specificity: C-Term

Purpose: Ghrelin preproprotein

Sequence: QDILWEEAKE APADK

Specificity: This antibody is expected to recognise all reported isoforms.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QDILWEEAKEAPADK, from the C Terminus of the protein sequence according to NP_057446.1, NP_001128413.1, NP_001128416.1, NP_001128417.1, NP_001128418.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows staining of trophoblast cytoplasm. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Colon. Recommended concentration: 2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Ghrelin antibody (ABIN185450)

Stoyanova, Wiertz, Rutten: "Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex." in: Regulatory peptides, Vol. 158, Issue 1-3, pp. 86-90, (2009) (PubMed).

Target Details for Ghrelin

Target: Ghrelin (GHRL)

Alternative Name: GHRL

Background: GHRL, MTLRP, ghrelin, ghrelin precursor, motilin-related peptide, preproghrelin, ghrelin/obestatin prepropeptide, obestatin, OTTHUMP00000207794, OTTHUMP00000207795, OTTHUMP00000207796, OTTHUMP00000207798, ghrelin, growth hormone secretagogue receptor liga

Gene ID: 51738

NCBI Accession: NP_057446, NP_001128413, NP_001128416, NP_001128417, NP_001128418

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour