Cofilin 2 antibody (C-Term)

Catalog No. ABIN185454

This anti-Cofilin 2 antibody is a Goat Polyclonal antibody detecting Cofilin 2 in WB, ELISA and IHC. Suitable for Human and Rat.

Quick Overview for Cofilin 2 antibody (C-Term) (ABIN185454)

Target: Cofilin 2 (CFL2)

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Cofilin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Cofilin 2 Antibody

Binding Specificity: C-Term

Purpose: Cofilin 2 (muscle)

Sequence: DIKDRSTLGE K

Specificity: This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DIKDRSTLGEK, from the C Terminus of the protein sequence according to NP_068733.1, NP_619579.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 18 kDa band observed in Human Skeletal Muscle lysates and in Rat Heart lysates (calculated MW of 18.7 kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cofilin 2

Target: Cofilin 2 (CFL2)

Alternative Name: CFL2

Background: Cofilin 2 (muscle) , HGNC:1875, CFL2, NEM7, cofilin 2

Gene ID: 1073

NCBI Accession: NP_068733, NP_619579

Pathways: Caspase Cascade in Apoptosis