CFC1 antibody (Internal Region)

Catalog No. ABIN185456

The Goat Polyclonal anti-CFC1 antibody has been validated for IHC and ELISA. It is suitable to detect CFC1 in samples from Mouse.

Quick Overview for CFC1 antibody (Internal Region) (ABIN185456)

Target: CFC1 (Cripto, FRL-1, Cryptic Family 1 (CFC1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CFC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CFC1 Antibody

Binding Specificity: Internal Region

Purpose: CFC1 / Cripto-1

Sequence: HFTGRYCEHD QRRSEC

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence HFTGRYCEHDQRRSEC, from the internal region of the protein sequence according to NP_115934.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration: 1-2 μg/mL.

Western Blot: Preliminary experiments gave an approx 55 kDa band in Human Breast, Placenta and Testis lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the ca

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CFC1

Target: CFC1 (Cripto, FRL-1, Cryptic Family 1 (CFC1))

Alternative Name: CFC1

Background: CFC1, HTX2, CRYPTIC, cripto, FRL-1, cryptic family 1, cripto-1, cryptic, cryptic gene, MGC133213, CR-1, FLJ77897

Gene ID: 55997

NCBI Accession: NP_115934