LIM Domain Binding 3 Protein antibody (Internal Region)

Catalog No. ABIN185471

The Goat Polyclonal anti-LIM Domain Binding 3 Protein antibody has been validated for WB, ELISA and IHC. It is suitable to detect LIM Domain Binding 3 Protein in samples from Human.

Quick Overview for LIM Domain Binding 3 Protein antibody (Internal Region) (ABIN185471)

Target: LIM Domain Binding 3 Protein (LDB3) (LIM Domain Binding 3 (LDB3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LIM Domain Binding 3 Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-LIM Domain Binding 3 Protein Antibody

Binding Specificity: Internal Region

Purpose: ZASP/ CYPHER / LDB3

Sequence: KSQNKPEDEA DE

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KSQNKPEDEADE, from the internal region of the protein sequence according to NP_009009.1, NP_001073583.1, NP_001073584.1, NP_001073585.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 6-7 μg/mL.

Western Blot: Western Blot experiments gave bands at approx 90 kDa and 35 kDa in Human Skeletal Muscle lysate after 1 μg/mL antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LIM Domain Binding 3 Protein

Target: LIM Domain Binding 3 Protein (LDB3) (LIM Domain Binding 3 (LDB3))

Alternative Name: LDB3

Background: LDB3, ZASP, LIM domain binding 3 , HGNC:15710, KIAA01613, KIAA0613, ORACLE, PDLIM6, PDZ and LIM domain 6, Z-band alternatively spliced PDZ-motif, CYPHER, FLJ35865, ldb3z1, ldb3z4

Gene ID: 11155

NCBI Accession: NP_009009, NP_001073583, NP_001073584, NP_001073585