Adenosine A2b Receptor antibody (C-Term)

Catalog No. ABIN185477

The Goat Polyclonal anti-Adenosine A2b Receptor antibody has been validated for ELISA, IHC, IF and FACS. It is suitable to detect Adenosine A2b Receptor in samples from Human.

Quick Overview for Adenosine A2b Receptor antibody (C-Term) (ABIN185477)

Target: Adenosine A2b Receptor (ADORA2B)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Adenosine A2b Receptor antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Adenosine A2b Receptor Antibody

Binding Specificity: C-Term

Purpose: Adenosine A2b Receptor

Sequence: CQADVKSGNG Q

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CQADVKSGNGQ, from the C Terminus of the protein sequence according to NP_000667.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules. Recommended concentration: 2-4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Adenosine A2b Receptor

Target: Adenosine A2b Receptor (ADORA2B)

Alternative Name: ADORA2B

Background: ADORA2B, adenosine A2b receptor, HGNC:264, ADORA2, netrin-1 receptor

Gene ID: 136

NCBI Accession: NP_000667

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process