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DARC antibody (N-Term)

Verified DARC Reactivity: Human WB, ELISA, IHC, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185480
  • Target See all DARC Antibodies
    DARC (Duffy Blood Group, Chemokine Receptor (DARC))
    Binding Specificity
    • 12
    • 12
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 39
    • 10
    • 6
    Human
    Host
    • 31
    • 8
    • 2
    • 1
    Goat
    Clonality
    • 35
    • 6
    • 1
    Polyclonal
    Conjugate
    • 24
    • 4
    • 4
    • 4
    • 3
    • 3
    This DARC antibody is un-conjugated
    Application
    • 28
    • 24
    • 8
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Purpose
    Duffy / FY / DARC
    Sequence
    HRAELSPSTE NSSQLDFED
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_001116423.1, NP_002027.2).
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1, NP_002027.2.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3

    Western Blot: Approx 37 kDa band observed in Human Liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    DARC (Duffy Blood Group, Chemokine Receptor (DARC))
    Alternative Name
    ACKR1 (DARC Products)
    Synonyms
    DARC antibody, FY antibody, ACKR1 antibody, CCBP1 antibody, CD234 antibody, Dfy antibody, GPD antibody, GpFy antibody, WBCQ1 antibody, AA162249 antibody, Ackr1 antibody, ESTM35 antibody, Duffy blood group, chemokine receptor antibody, atypical chemokine receptor 1 (Duffy blood group) antibody, atypical chemokine receptor 1 antibody, DARC antibody, ACKR1 antibody, Ackr1 antibody
    Background
    FY, DARC, HGNC:4035, Duffy blood group, CCBP1, GPD, CTA-134P22.3, CD234, FY, OTTHUMP00000060081, Duffy blood group, chemokine receptor, Duffy blood group antigen, Glycoprotein D, Dfy, GpFy, WBCQ1, Fy glycoprotein, OTTHUMP00000035293, OTTHUMP00000035294
    Gene ID
    2532
    NCBI Accession
    NP_001116423, NP_002027
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