CNR1 antibody (Internal Region)

Catalog No. ABIN185484

This anti-CNR1 antibody is a Goat Polyclonal antibody detecting CNR1 in WB and ELISA. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for CNR1 antibody (Internal Region) (ABIN185484)

Target: CNR1 (Cannabinoid Receptor 1 (CNR1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CNR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CNR1 Antibody

Binding Specificity: Internal Region

Purpose: Cannabinoid Receptor 1

Sequence: HTSEDGKVQV TRPDQ

Specificity: This antibody is expected to recognise both reported isoforms. Isoform a (NP_057167.2, NP_001153732.1, NP_001153731.1, NP_001153730.1, NP_001153698.1) and isoform b (NP_149421.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HTSEDGKVQVTRPDQ, from the internal region of the protein sequence according to NP_057167.2, NP_001153732.1, NP_001153731.1, NP_001153730.1, NP_001153698.1, NP_149421.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa (non-glycosylated) and 65 kDa (glycosylated) bands observed in lysates of cell line HeLa (calculated MW of 52.9 kDa according to NP_057167.2,). Both bands were successfully blocked by incubation with the immunizing peptide, Reco

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-CNR1 antibody (ABIN185484)

Fügedi, Molnár, Rigó, Schönléber, Kovalszky, Molvarec: "Increased placental expression of cannabinoid receptor 1 in preeclampsia: an observational study." in: BMC pregnancy and childbirth, Vol. 14, pp. 395, (2015) (PubMed).

Target Details for CNR1

Target: CNR1 (Cannabinoid Receptor 1 (CNR1))

Alternative Name: CNR1

Background: Cannabinoid receptor 1 (brain), CNR1, HGNC:2159, CANN6, CB-R, CB1, CB1A, CB1K5, CNR, central cannabinoid receptor, RP1-23D17.1, CB1R, cannabinoid receptor 1 OTTHUMP00000016838, OTTHUMP00000016839

Gene ID: 1268, 12801, 25248

NCBI Accession: NP_057167, NP_001153732, NP_001153731, NP_001153730, NP_001153698, NP_149421

Pathways: Feeding Behaviour