KPNB1 antibody (C-Term)

Catalog No. ABIN185516

The Goat Polyclonal anti-KPNB1 antibody is suitable to detect KPNB1 in samples from Human and Rat. It has been validated for WB, IHC, ELISA, FACS and IF.

Quick Overview for KPNB1 antibody (C-Term) (ABIN185516)

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This KPNB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-KPNB1 Antibody

Binding Specificity: C-Term

Purpose: Karyopherin (importin) beta 1

Sequence: RRSKTNKAKT LAT

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RRSKTNKAKTLAT, from the C Terminus of the protein sequence according to NP_002256.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 90-95- kDa band observed in lysates of cell lines A431, Daudi, HEK293, HeLa, Jurkat, Kelly and KNRK (calculated MW of 97.2 kDa according to Human NP_002256.2 and Rat NP_058759.2). Recommended concentration: 0.01-0.1 μg/mL. Primary incu

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KPNB1

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Alternative Name: KPNB1

Background: KPNB1, karyopherin (importin) beta 1, HGNC:6400, IMB1, IPOB, Impnb, MGC2155, MGC2156, MGC2157, NTF97, importin 90, importin beta-1 subunit, karyopherin beta 1, nuclear factor p97, KapB1, IPO1

Gene ID: 3837, 16211, 24917

NCBI Accession: NP_002256

Pathways: Protein targeting to Nucleus