FZD8 antibody (C-Term)

Catalog No. ABIN185522

The Goat Polyclonal anti-FZD8 antibody has been validated for WB, ELISA, IHC, IF and IEM. It is suitable to detect FZD8 in samples from Human.

Quick Overview for FZD8 antibody (C-Term) (ABIN185522)

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)

Grade: Verified

Product Details anti-FZD8 Antibody

Binding Specificity: C-Term

Purpose: FZD8 / frizzled 8

Sequence: SYPKQMPLSQ V

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 3 μg/mL.

Western Blot: Approx 75 kDa band observed in Human Pancreas lysates (calculated MW of 73.3 kDa according to NP_114072.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse: Zhao B e

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: This antibody has been successfully used in IF on Mouse: Zhao B et al. (2019) PMID: 21321602.

Additional validation: This antibody was successfully used in Electron microscopy (immunogold staining):

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FZD8

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Alternative Name: FZD8

Background: FZD8, frizzled homolog 8 (Drosophila) , HGNC:4046, FZ-8, hFZ8 , frizzled (Drosophila) homolog 8, frizzled 8

Gene ID: 8325, 14370

NCBI Accession: NP_114072

Pathways: WNT Signaling