PDE4D antibody (C-Term)

Catalog No. ABIN185530

This Goat Polyclonal antibody specifically detects PDE4D in WB, ELISA, IHC and EIA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for PDE4D antibody (C-Term) (ABIN185530)

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PDE4D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Grade: Verified

Product Details anti-PDE4D Antibody

Binding Specificity: C-Term

Purpose: PDE4D

Sequence: QPEACVIDDR SPDT

Specificity: This antiobody is expected to recognize all reported isoforms.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_001184151.1, NP_001184150.1, NP_006194.2, NP_001098101.1, NP_001184152.1, NP_001159371.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 70+80+90 kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. Calculated

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol. 2010 Jan,47(4):678-84., PMID: 19939455.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PDE4D antibody (ABIN185530)

Bazhin, Kahnert, Kimpfler, Schadendorf, Umansky: "Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells." in: Molecular immunology, Vol. 47, Issue 4, pp. 678-84, (2010) (PubMed).

Target Details for PDE4D

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Alternative Name: PDE4D

Background: PDE4D, phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila), HGNC:8783, DPDE3, HSPDE4D, PDE4DN2, STRK1, cAMP-specific phosphodiesterase 4D, cAMP-specific phosphodiesterase PDE4D6, dunce-like phosphodiesterase E3, phosphodie

Gene ID: 5144

NCBI Accession: NP_001184151, NP_001184150, NP_006194, NP_001098101, NP_001184152, NP_001159371

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling