DCDC2 antibody (Internal Region)

Catalog No. ABIN185538

The Goat Polyclonal anti-DCDC2 antibody (ABIN185538) specifically detects DCDC2 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DCDC2 antibody (Internal Region) (ABIN185538)

Target: DCDC2 (Doublecortin Domain Containing 2 (DCDC2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This DCDC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-DCDC2 Antibody

Binding Specificity: Internal Region

Purpose: DCDC2

Sequence: DGEKANKDAE QKED

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DGEKANKDAEQKED, from the internal region of the protein sequence according to NP_057440.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in select neurons. Recommended concentration, 5-10 μg/mL. An anonymous customer found positive results in IHC on Rat retinal tissue. An anonymous customer found posi

Western Blot: Full length recombinant human DCDC2 expressed in COS7 (Lane A), non-transfectant COS7 (lane B). Approx 52 kDa band observed (calculated MW of 52.8 kDa according to NP_057440.1). Data kindly provided by Wang and LoTurco, University of Connecti

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DCDC2

Target: DCDC2 (Doublecortin Domain Containing 2 (DCDC2))

Alternative Name: DCDC2

Background: DCDC2, doublecortin domain containing 2, HGNC:18141, KIAA1154, RU2, RU2S, DCDC2A

Gene ID: 51473

NCBI Accession: NP_057440