PPIA antibody (Internal Region)

Catalog No. ABIN185542

This Goat Polyclonal antibody specifically detects PPIA in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PPIA antibody (Internal Region) (ABIN185542)

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PPIA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PPIA Antibody

Binding Specificity: Internal Region

Purpose: Cyclophilin A / PPIA

Sequence: ERFGSRNGKT SKK

Specificity: This product is expected to also recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ERFGSRNGKTSKK, from the internal region of the protein sequence according to NP_066953.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx.18 kDa band observed in Human Lymph Node lysates (calculated MW 18.0 kDa according to NP_066953.1). Recommended Concetration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPIA

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Alternative Name: PPIA

Background: PPIA, peptidylprolyl isomerase A (cyclophilin A) , HGNC:9253, CYPA, CYPH, MGC12404 , T cell cyclophilin, cyclosporin A-binding protein, peptidyl-prolyl cis-trans isomerase A, peptidylprolyl isomerase A, rotamase, MGC117158, MGC23397, PPIase A

Gene ID: 5478

NCBI Accession: NP_066953