TIM3 antibody (Internal Region)

Catalog No. ABIN185571

This anti-TIM3 antibody is a Goat Polyclonal antibody detecting TIM3 in FACS, WB, ELISA and IF. Suitable for Human.

Quick Overview for TIM3 antibody (Internal Region) (ABIN185571)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TIM3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-TIM3 Antibody

Binding Specificity: Internal Region

Purpose: TIM3 / HAVCR2

Sequence: KWYSHSKEKI QN

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KWYSHSKEKIQN, from the internal region of the protein sequence according to NP_116171.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in lysates of cell lines A549 and HepG2 and in preliminary testing of Jurkat and U937 cell lysate (calculated MW of 33.4 kDa according to NP_116171.3). Recommended concentration:0.1-1 μg/mL. Primary incubation 1 hou

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus and plasma membrane of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended con

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: HAVCR2

Target Type: Virus

Background: HAVCR2, TIM3, hepatitis A virus cellular receptor 2, FLJ14428, KIM-3, TIMD3, Tim-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, kidney injury molecule-3

Gene ID: 84868

NCBI Accession: NP_116171

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints