PDE4B antibody (C-Term)

Catalog No. ABIN185574

This anti-PDE4B antibody is a Goat Polyclonal antibody detecting PDE4B in WB, ELISA and IHC. Suitable for Human and Mouse.

Quick Overview for PDE4B antibody (C-Term) (ABIN185574)

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PDE4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PDE4B Antibody

Binding Specificity: C-Term

Purpose: Phosphodiesterase 4B

Sequence: DIDIATEDKS PVDT

Specificity: This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DIDIATEDKSPVDT, from the C Terminus of the protein sequence according to NP_002591.2, NP_001032418.1, NP_001032416.1, NP_001032417.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 85 kDa band observed in Mouse Brain lysates (calculated MW of 83.3 kDa according to Human NP_002591.2 and 82.1 kDa according to Mouse NP_062814.2). In transfected HEK293 transiently expressing Human PDE4B a band of approx. 85 kDa is obse

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PDE4B

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Alternative Name: PDE4B

Background: PDE4B, phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila), RP5-876C12.1, DKFZp686F2182, DPDE4, MGC126529, PDEIVB , OTTHUMP00000046896, cAMP-specific 3',5'-cyclic phosphodiesterase 4B, dunce-like phosphodiesterase E4, phos

Gene ID: 5142

NCBI Accession: NP_002591, NP_001032418, NP_001032416, NP_001032417

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction