DCP1A antibody (C-Term)

Catalog No. ABIN185579

The Goat Polyclonal anti-DCP1A antibody is suitable to detect DCP1A in samples from Human. It has been validated for WB and ELISA.

Quick Overview for DCP1A antibody (C-Term) (ABIN185579)

Target: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DCP1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DCP1A Antibody

Binding Specificity: C-Term

Purpose: DCP1A

Sequence: QVLTKNKDNH N

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QVLTKNKDNHN, from the C Terminus of the protein sequence according to NP_060873.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in lysates of cell line A431 and HeLa (calculated MW of 63.3 kDa according to NP_060873.3). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DCP1A

Target: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Alternative Name: DCP1A

Background: DCP1A, decapping enzyme , HSA275986, Nbla00360, SMAD4IP1, SMIF, Smad4-interacting transcriptional co-activator, decapping enzyme hDcp1a, putative protein product of Nbla00360, transcription factor SMIF

Gene ID: 55802, 75901

NCBI Accession: NP_060873