DDX6 antibody (C-Term)

Catalog No. ABIN185580

This anti-DDX6 antibody is a Goat Polyclonal antibody detecting DDX6 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for DDX6 antibody (C-Term) (ABIN185580)

Target: DDX6 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DDX6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-DDX6 Antibody

Binding Specificity: C-Term

Purpose: DEAD-box protein 6

Sequence: AEYHSEPVED EKP

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-AEYHSEPVEDEKP, from the C Terminus of the protein sequence according to NP_004388.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Thymus. Recommended concentration: 5 μg/mL.

Western Blot: Approx 50 kDa band observed in lysates of cell lines Daudi, Jurkat and HepG2 (calculated MW of 53.2 kDa according to NP_004388.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DDX6

Target: DDX6 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6))

Alternative Name: DDX6

Background: DEAD-box protien 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DDX6, HLR2, P54, RCK, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD),

Gene ID: 1656

NCBI Accession: NP_004388

Pathways: Ribonucleoprotein Complex Subunit Organization