CYP3A4 antibody (Internal Region)

Catalog No. ABIN185606

The Goat Polyclonal anti-CYP3A4 antibody has been validated for IHC, ELISA and FACS. It is suitable to detect CYP3A4 in samples from Human.

Quick Overview for CYP3A4 antibody (Internal Region) (ABIN185606)

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CYP3A4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-CYP3A4 Antibody

Binding Specificity: Internal Region

Purpose: CYP3A4

Sequence: KESRLEDTQK HR

Specificity: This antibody is expected to recognise both reported isoforms (NP_059488.2, NP_001189784.1) and it is not expected to cross-react with 3A5, 3A7 ot 3A43.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KESRLEDTQKHR

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5-7 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYP3A4

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Alternative Name: CYP3A4

Background: CYP3A4, cytochrome P450, family 3, subfamily A, polypeptide 4 , HGNC:2637, CP33, CP34, CYP3A, CYP3A3, HLP, NF-25, P450C3, P450PCN1, P450-III, steroid inducible, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3, cytochrome P450, subfami

Gene ID: 1576

NCBI Accession: NP_059488, NP_001189784

Pathways: Steroid Hormone Biosynthesis