Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This CYP3A43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-270 amino acids from the Central region of human CYP3A43.
CYP3A4
Reactivity: Human
WB, IHC, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
IF: 1:10~50. WB: 1:2000. FC: 1:10~50
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Okamatsu, Kawakami, Komatsu, Kitazawa, Uno, Teraoka: "Functional expression and comparative characterization of four feline P450 cytochromes using fluorescent substrates." in: Xenobiotica; the fate of foreign compounds in biological systems, Vol. 47, Issue 11, pp. 951-961, (2017) (PubMed).
Target
CYP3A4
(Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))
CYP3A43 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, the protein unknown whether the enzyme is also involved in xenobiotic metabolism.