SMAD9 antibody (Internal Region)

Catalog No. ABIN185609

The Goat Polyclonal anti-SMAD9 antibody has been validated for WB, IHC and ELISA. It is suitable to detect SMAD9 in samples from Human.

Quick Overview for SMAD9 antibody (Internal Region) (ABIN185609)

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SMAD9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SMAD9 Antibody

Binding Specificity: Internal Region

Purpose: SMAD9

Sequence: HSEPLMPHNA TYPD

Specificity: This antibody is not expected to cross-react with SMAD1 and SMAD5.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HSEPLMPHNATYPD, from the internal region of the protein sequence according to NP_005896.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Skin lysates (calculated MW of 48.6 kDa according to NP_005896.1). Recommended concentration: 0.3-1.0 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SMAD9

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Alternative Name: SMAD9

Background: SMAD9, SMAD, mothers against DPP homolog 9 (Drosophila)] , HGNC:6774, MADH6, MADH9, SMAD8A, SMAD8B , MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, MAD, mothers against decapentaplegic homolog

Gene ID: 4093

NCBI Accession: NP_005896