PIP antibody (Internal Region)

Catalog No. ABIN185621

The Goat Polyclonal anti-PIP antibody has been validated for WB, IHC and ELISA. It is suitable to detect PIP in samples from Human.

Quick Overview for PIP antibody (Internal Region) (ABIN185621)

Target: PIP (Prolactin-Induced Protein (PIP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PIP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PIP Antibody

Binding Specificity: Internal Region

Purpose: GCDFP15 / PIP

Sequence: GANKAQDNTR K

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GANKAQDNTRK, from the internal region of the protein sequence according to NP_002643.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows staining of luminal epithelial cells of the lactiferous duct. Recommended concentration: 2-3 μg/mL.

Western Blot: Approx 14 kDa band observed in lysates of cell line A431 (calculated MW of 16.6 kDa according to NP_002643.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PIP

Target: PIP (Prolactin-Induced Protein (PIP))

Alternative Name: PIP

Background: PIP, GCDFP15, prolactin-induced protein , HGNC:8993, GCDFP-15, GP17, GPIP4, prolactin-inducible protein, gross cystic disease fluid protein 15

Gene ID: 5304

NCBI Accession: NP_002643