AKR1C4 antibody (N-Term)

Catalog No. ABIN185625

This anti-AKR1C4 antibody is a Goat Polyclonal antibody detecting AKR1C4 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for AKR1C4 antibody (N-Term) (ABIN185625)

Target: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKR1C4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-AKR1C4 Antibody

Binding Specificity: N-Term

Purpose: AKR1C4

Sequence: DPKYQRVELN DGH

Specificity: This antibody may cross-react with AKR1C1

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DPKYQRVELNDGH-C, from the N Terminus of the protein sequence according to NP_001809.2

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows staining of distinct structures in the cytoplasm of hepatocytes. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx. 35 kDa band observed in Human Liver lysates (calculated MW of 37.1 kDa according to NP_001809.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AKR1C4

Target: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Alternative Name: AKR1C4

Background: AKR1C4, aldo-keto reductase family 1, member C4, HGNC:387, 3-alpha-HSD, C11, CDR, CHDR, DD4, HAKRA, MGC22581, 3-alpha hydroxysteroid dehydrogenase, type I , 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase 4, 3-alpha-hydroxysteroid dehydroge

Gene ID: 1109

NCBI Accession: NP_001809

Pathways: Steroid Hormone Biosynthesis